Journal of Allergy and Clinical Immunology





① 分析66名健康婴幼儿和63名特应性皮炎(AD)患儿的肠道菌群组成;② 6月龄婴幼儿的肠道菌群组成与喂养方式相关,母乳喂养/混合喂养的婴幼儿中均存在两种肠型;③ AD患儿粪便中的细菌数量低于对照组,虽无特殊的门类直接与AD相关,但免疫发育相关的功能性基因有差异;④ 宏基因组分析表明,AD患儿免疫发育迟缓与菌群中某些基因较少有关,包括氧化磷酸化、多种信号通路(PI3K-Akt、雌激素、NOD样受体)、黏蛋白降解菌定殖相关的抗原加工呈递。

肠道菌群 特应性皮炎 婴幼儿 免疫


Perturbations of the gut microbiome genes in infants with atopic dermatitis according to feeding type


Abstract & Authors展开

Perturbations of the infant gut microbiota can shape the development of the immune system and link to the risk of allergic diseases.To understand the role of the gut microbiome in atopic dermatitis, the metagenome of the infant gut microbiome was analyzed according to feeding types.The composition of gut microbiota was analyzed in fecal samples from 129 infants (6-month-old) by pyrosequencing, including 66 healthy infants and 63 infants with atopic dermatitis. The functional profile of the gut microbiome was analyzed by whole metagenome sequencing (20 controls and 20 AD). In addition, the total number of bacteria in the feces was determined by real-time PCR.The gut microbiome of 6-month-old infants was different by feeding types, and two microbiota groups (Bifidobacterium dominated and Escherichia/Veillonella dominated group) were found in breast-fed and mixed-fed infants. The bacterial cell amounts in the feces were lower in infants with AD than in controls. Although no specific taxa directly correlated with AD in 16S rRNA gene results, whole metagenome analysis revealed differences in functional genes related to immune development. The reduction of genes for oxidative phosphorylation, PI3K-Akt signaling, estrogen signaling, NOD-like receptor signaling, and antigen processing and presentation induced by reduced colonization of mucin-degrading bacteria (Akkermansia muciniphila, Ruminocccus gnavus, and Lachnospiraceae bacterium 2_1_58FAA) was significantly associated with stunted immune development in the AD group compared to the control group (P < .05).Alterations in the gut microbiome may be associated with atopic dermatitis due to different bacterial genes that can modulate host immune cell function.

All Authors:
Min-Jung Lee, Mi-Jin Kang, So-Yeon Lee, Eun Lee, Kangjin Kim, Sungho Won, Dong In Suh, Kyung Won Kim, Youn Ho Sheen, Kangmo Ahn, Bong-Soo Kim, Soo-Jong Hong

First Authors:
Min-Jung Lee

Bong-Soo Kim, Soo-Jong Hong