克罗恩病患者呼气中的挥发性代谢物与肠道菌群相关

Analytica Chimica Acta

3/30/18Article

4.95

影响因子

原标题:呼吸中的挥发性代谢物与克罗恩病患者的肠道菌群密切相关

① 纳入68名克罗恩病(CD)患者,收集粪便和呼吸样本,分析活动期和静止期的CD患者呼气中挥发性代谢物与肠道菌群的关系;② 挥发性代谢物与菌群显著相关,且活动期和静止期疾病表现出的相关性不同;③ 其中乙酸、戊酸、丙酸与活动期和静止期菌群均相关;④ 乙酸、丙酸与厚壁菌门中的某些成员及双歧杆菌显著相关,与静止期菌群相关性更强;⑤ 活动期CD患者中,脆弱拟杆菌和戊、辛烷显著相关,苯酚与拟杆菌属、普氏菌属显著相关。

克罗恩病

图片

Title:
Volatile metabolites in breath strongly correlate with gut microbiome in CD patients

DOI:
10.1016/j.aca.2018.03.046

Abstract & Authors展开

Abstract:
Microbiota composition and its metabolic capacity are very important for host health. Evidence suggests that gut microbiome is involved in the metabolites production by host-microbiome interaction. These metabolites can be absorbed in blood and excreted in exhaled air. Although, profiles of gut microbiota and exhaled metabolites were associated with gastrointestinal diseases, a direct link between them has not yet been investigated. The aim of the study was to investigate the relation between volatiles in breath and gut microbiome in active and quiescent Crohn's disease (CD) via a multivariate statistical approach. Canonical correlation analysis (CCA) was used to assess the relation between exhaled metabolites and faecal bacterial species. From 68 CD patients, 184 repeated faecal and breath samples were collected (92 active and 92 quiescent disease). The microbiota composition was assessed by the pyrosequencing of the 16 S rRNA V1-V3 gene region and breath metabolites by gas chromatography mass spectrometry. In active disease, CCA analysis identified 18 metabolites significantly correlated with 19 faecal bacterial taxa (R = 0.91 p-value 3.5*10-4). In quiescent disease 17 volatile metabolites were correlated with 17 bacterial taxa (R = 0.96 p-value 2.8*10-4). Nine metabolites and three bacteria taxa overlapped in active and inactive CD. This is the first study that shows a significant relation between gut microbiome and exhaled metabolites, and was found to differ between active and quiescent CD, indicating various underlying mechanisms. Unravelling this link is essential to increase our understanding on the functional effects of the microbiome and may provide new leads for microbiome-targeted intervention.

All Authors:
Agnieszka Smolinska, Danyta I Tedjo, Lionel Blanchet, Alexander Bodelier, Marieke J Pierik, Ad AM Masclee, Jan Dallinga, Paul HM Savelkoul, Daisy MAE Jonkers, John Penders, Frederik-Jan van Schooten

First Authors:
Agnieszka Smolinska

Correspondence:
Agnieszka Smolinska

查看PDF原文

关闭

用微信扫描上面二维码,验证身份

提醒:您需要先关注微信公众号“热心肠先生”,才能验证通过!

 验证成功,3秒后跳转

已超时,请 重试

 

已完成

请稍等...

提示
提示